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Dr Ali Ryan

Lecturer

About

Dr Ryan did his degree at Imperial College during which he spent a sandwich year designing high throughput screening assays for GlaxoSmithKline. His PhD was with Prof. Stephen Curry at Imperial College using structural biology to understand the transport of drugs around the body. He then moved to take up a postdoctoral position with Prof. Edith Sim in Oxford University studying novel antibacterial targets in M. tuberculosis and Ps. aeruginosa. In 2012 he moved to Kingston University to run the newly opened biotechnology laboratory.

Qualifications

  • PhD
  • BSc

Research

My primary research interests are in the identification of novel targets for antimicrobial therapeutics and the development of novel strategies to overcome bacterial infection. My lab uses a range of molecular and microbiology techniques alongside recombinant protein technology, enzymatic assays and X-ray crystallography to identify and characterise novel targets. In early 2012 I moved to Kingston University to establish the state of the art biotechnology laboratory. Our lab acts as a core facility in the newly formed faculty of science engineering and computing. Alongside my own research, my job is to provide staff with the expertise and support in molecular biology and to produce recombinant protein from a range of organisms from bacteria to humans. I am then involved with characterising these proteins and eventually crystallising them. All of our projects are also supported by an international network of collaborations with researchers across the UK and Europe e.g. Dr Gail Preston at Oxford University. The focus of my research is the identification, characterisation and exploitation of novel antimicrobial targets. Currently my lab focuses on two major projects involving targets in P. aeruginosa and M. tuberculosis. In P. aeruginosa we focus on the azoreductase family of enzymes that are found ubiquitously in bacteria. Although they lack a clear physiological function, they are known to prevent mammalian infection in P. aeruginosa. Work in my lab has shown that they are important determinants of antibiotic sensitivity in the bacteria and we are now investigating how these enzymes control such diverse phenotypes. In M. tuberculosis our investigations centre round developing inhibitors of genes from the cholesterol metabolism pathway. We are using biophysical and high throughput screening techniques to identify inhibitors of a novel target for anti-tubercular agents, which was initially identified in our lab. These initial hits have now been validated with the help of researchers at UCL who have shown they inhibit the growth of M. tuberculosis on cholesterol. I also advise a range of other projects including ones focusing on; heart disease, cancer, diabetes and optics of the eye. 

Publications

Number of items: 16.

Article

Sim, Edith and Ryan, Ali (2017) Drug metabolism and antibiotic resistance in micro-organisms. British Journal of Pharmacology, ISSN (print) 0007-1188 (Epub Ahead of Print)

Eleftheriadou, Olga, Boguslavskyi, Andrii, Longman, Michael R., Cowan, Jonathan, Francois, Asvi, Heads, Richard J., Wadzinski, Brian E., Ryan, Ali, Shattock, Michael J. and Snabaitis, Andrew K. (2017) Expression and regulation of type 2A protein phosphatases and alpha4 signalling in cardiac health and hypertrophy. Basic Research in Cardiology, 112(37), pp. 1-18. ISSN (print) 0300-8428

Ryan, Ali, Polycarpou, Elena, Lack, Nathan A., Evangelopoulos, Dimitrios, Sieg, Christian, Halman, Alice, Bhakta, Sanjib, Eleftheriadou, Olga, McHugh, Timothy D., Keany, Sebastian, Lowe, Edward D., Ballet, Romain, Abuhammad, Areej, Jacobs Jr., William R., Ciulli, Alessio and Sim, Edith (2017) Investigation of the mycobacterial enzyme HsaD as a potential novel target for anti-tubercular agents using a fragment-based drug design approach. British Journal of Pharmacology, ISSN (print) 0007-1188 (Epub Ahead of Print)

da Silva, RAG, Churchward, CP, Karlyshev, AV, Eleftheriadou, O, Snabaitis, AK, Longman, MR, Ryan, A and Griffin, R (2017) The role of apolipoprotein N-acyl transferase, Lnt, in the lipidation of Factor H binding protein of ' Neisseria meningitidis ' strain MC58 and its potential as a drug target. British Journal of Pharmacology, 174(14), pp. 2247-2260. ISSN (print) 0007-1188

Ryan, Ali (2016) Azoreductases in drug metabolism. British Journal of Pharmacology, ISSN (online) 1476-5381 (Epub Ahead of Print)

Crescente, Vincenzo, Holland, Sinead M., Kashyap, Sapna, Polycarpou, Elena, Sim, Edith and Ryan, Ali (2016) Identification of novel members of the bacterial azoreductase family in Pseudomonas aeruginosa. Biochemical Journal, 473(5), pp. 549-558. ISSN (print) 0264-6021

Ryan, Ali, Kaplan, Elise, Nebel, Jean-Christophe, Polycarpou, Elena, Crescente, Vincenzo, Lowe, Edward, Preston, Gail M and Sim, Edith (2014) Identification of NAD(P)H Quinone Oxidoreductase Activity in Azoreductases from P. aeruginosa: Azoreductases and NAD(P)H Quinone Oxidoreductases Belong to the Same FMN-Dependent Superfamily of Enzymes. PloS ONE, 9(6), e98551. ISSN (online) 1932-6203

Mahendiran, K., Elie, C., Nebel, J-C., Ryan, A. and Pierscionek, B.K. (2014) Primary sequence contribution to optical function of the eye lens. Scientific Reports, 4, p. 5195. ISSN (online) 2045-2322

Ryan, Ali, Keany, Sebastian, Eleftheriadou, Olga, Ballet, Romain, Cheng, Hung-Yuan and Sim, Edith (2014) Research Letter: Mechanism-based inhibition of HsaD: a C-C bond hydrolase essential for survival of M. tuberculosis in macrophage. FEMS Microbiology Letters, 350(1), pp. 42-47. ISSN (print) 0378-1097

Egleton, James E., Thinnes, Cyrille C., Seden, Peter T., Laurieri, Nicola, Lee, Siu Po, Hadavizadeh, Kate S., Measures, Angelina R., Jones, Alan M., Thompson, Sam, Varney, Amy, Wynne, Graham M., Ryan, Ali, Sim, Edith and Russell, Angela J. (2014) Structure-activity relationships and colorimetric properties of specific probes for the putative cancer biomarker human arylamine [Nu]-acetyltransferase 1. Bioorganic & Medicinal Chemistry, 22(11), pp. 3030-3054. ISSN (print) 0968-0896

Ryan, Ali, Kaplan, Elise, Laurieri, Nicola, Lowe, Edward and Sim, Edith (2011) Activation of nitrofurazone by azoreductases: multiple activities in one enzyme. Scientific Reports, 1, ISSN (online) 2045-2322

Ryan, Ali J., Chung, Chun-wa and Curry, Stephen (2011) Crystallographic analysis reveals the structural basis of the high-affinity binding of iophenoxic acid to human serum albumin. BMC Structural Biology, 11(18), ISSN (online) 1472-6807

Ryan, Ali J., Ghuman, Jamie, Zunszain, Patricia A., Chung, Chun-wa and Curry, Stephen (2011) Structural basis of binding of fluorescent, site-specific dansylated amino acids to human serum albumin. Journal of Structural Biology, 174(1), pp. 84-91. ISSN (print) 1047-8477

Ryan, Ali, Laurieri, Nicola, Westwood, Isaac, Wang, Chan-Ju, Lowe, Edward and Sim, Edith (2010) A novel mechanism for azoreduction. Journal of Molecular Biology, 400(1), pp. 24-37. ISSN (print) 0022-2836

Conference or Workshop Item

Eleftheriadou, Olga, Boguslavskyi, Andrii, Ryan, Ali, Shattock, Michael J, Wadzinski, Brian E and Snabaitis, Andrew K (2015) Expression of type 2A protein phosphatases in cardiac health and disease. In: 33rd Meeting of the International Society for Heart Research-European Section; 01 - 04 Jul 2015, Bordeaux, France.

Eleftheriadou, O, Longman, MR, Boguslavskyi, A, Ryan, A, Wadzinski, BE, Shattock, MJ and Snabaitis, AK (2014) Expression of type 2A protein phosphatases in cardiac health and disease. In: 2014 Autmun Meeting British Society for Cardiovascular Research; 08 - 09 Sep 2014, Reading, U.K..

This list was generated on Sun Nov 19 04:32:48 2017 GMT.
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